Gladys Maria V. Pangga
1College of Veterinary Medicine, University of the Philippines Los Baños
Title: ANTIMICROBIAL RESISTANCE PROFILE OF Escherichia coli ISOLATED FROM PORK IN PUBLIC MARKETS IN LAGUNA
Biography
Biography: Gladys Maria V. Pangga
Abstract
A total of 27 E. coli were isolated from pork samples purchased from various public markets in Laguna. Methods were adapted from the NARMS (2006) protocol and isolates were identified using traditional biochemical methods and confirmed using RapID™ ONE kit (Remel, ThermoFisher, USA). Minimum inhibitory concentrations (MICs) against against 9 critically important antimicrobials were determined using the Clinical and Laboratory Standards Institute (CLSI, 2017) broth microdilution procedure using ATCC 25922 as control. Out of the 19 public markets, 17 were positive for presence of E. coli in pork chops. Among the 27 recovered E. coli isolates, two (7.4%) were suspected as E. coli O157:H7. Resistance were most frequently observed in tetracycline (96%) and doxycycline (93%), followed by cephalothin (89%), trimethoprim (89%), ampicillin (78%), chloramphenicol (70%) and ciprofloxacin (48%); whereas fewer resistant isolates were detected against kanamycin (4%) and gentamicin (4%), with MICs ranging from 4-64 µg/ml. All isolates were resistant to at least three antimicrobials indicating a 100% multidrug resistant bacterial population. These results suggest pork is an important reservoir of antimicrobial resistant E. coli exposing the public to potential health hazard which may facilitate dissemination of resistant genes to commensal and enteric bacteria through the food chain.
Keywords: antimicrobial resistance, Escherichia coli, antibiotic sensitivity, minimum inhibitory concentration, broth microdilution, pork