Analytical strategies for Antimicrobials & Antibiotics


The analysis of antibiotic susceptibility disks by high-pressure liquid chromatography (HPLC) methods are presented for the potency determination of mecillinam, ampicillin, carbenicillin, and cephalotjiin alone and in various combinations. Good agreement between HPLC and microbiological data is observed for potency determinations with recoveries of greater than 95%. Relative standard deviations of less than 2% are recorded for every HPLC method. HPLC methods offer improved accuracy and greater precision in comparison to the quality microbiological methods of study for susceptibility disks. the foremost popular analytical methods for the determination of beta-lactams are the chromatographic ones, including high performance liquid-chromatography (HPLC) and thin layer chromatography (TLC).

HPLC offers high sensitivity and separation efficiency, establishing itself because the first choice method for the analysis of beta-lactams; however it's expensive and requires sophisticated equipment. TLC may be a less costly and fewer complicated chromatographic procedure, which may be successfully used, within the preliminary screening of pharmaceutical substances. In modern analysis, TLC is typically used as a separation method, which establishes the presence or absence of beta-lactam antibiotics above an outlined level of concentration.

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